cGMP Direct Immunoassay Kit (Colorimetric)

cGMP Direct Immunoassay Kit (Colorimetric)

Size

100 assays

Catalog no.

K372-100

Price

480 EUR

Buy
NameValue
Kit's other name none
Category cGMP
Storage condition -20°C
Applications Inquire
Shipping condition gel pack
Maximum time can be stored 12 months
Species reactivity see datasheet
Properties Colorimetric assays or detection use UV absorption or enzymatic color reaction.
Samples tested Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Highlights • Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: The kit can detect 0.04 :10 pmol/well (0.008 : 2 µM) cGMP samples.
Contents • 10X cGMP Assay Buffer • Standard cGMP (10 nmol) • Neutralizing Buffer • Acetylating Reagent A • Acetylating Reagent B • Anti-cGMP pAb/BSA • HRP Developer • Protein G Coated Plate
Kit's benefits • Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • The kit provides a new acetylation procedure to improve detection sensitivity significantly
Kit's description Adenosine and guanosine 3’,5’-cyclic monophosphate (cAMP and cGMP) are important “second messengers” involved in many physiological processes. BioVision’s cGMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples. The kit utilizes the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cGMP in samples. The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.